Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Period circadian regulator 3 (PER3) enhances sensitivity to radiotherapy in gastric cancer via Wnt/β-catenin pathway

Zhiyi Shangguan¹, Qian Zhang², Tian Luan¹, Hongtao Hu³

¹Department of Radiation Therapy Technology Center, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150040, China; ²Department of Radiation Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150040, China; ³Office of President, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150040, China.

For correspondence:- Hongtao Hu Email: 2115@hrbmu.edu.cn Tel:+8645186298666

Accepted: 28 May 2023 Published: 30 June 2023

Citation: Shangguan Z, Zhang Q, Luan T, Hu H. Period circadian regulator 3 (PER3) enhances sensitivity to radiotherapy in gastric cancer via Wnt/β-catenin pathway. Trop J Pharm Res 2023; 22(6):1205-1210 doi: 10.4314/tjpr.v22i6.9

© 2023 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate a novel radiotherapeutic biomarker for gastric cancer (GC) treatment.

Methods: Radioresistant gastric cancer cells (NCI-N87-RR and MKN45-RR) were established using Gy. Protein levels were determined using western blots. Clone formation was evaluated and cell viability assessed by cell counting kit-8 (CCK-8). Apoptosis was determined by flow cytometry.

Results: Period circadian regulator 3 (PER3) was down-regulated in both cell lines (NCI-N87-RR and MKN45-RR). Moreover, PER3 over-expression enhanced sensitivity to radiation in radioresistant gastric cancer cells. Cell proliferation was inhibited, while PER3 promoted cell apoptosis. Furthermore, PER3 over-expression suppressed β-catenin and cellular myelocytomatosis oncogene (c-myc) and enhanced axin protein level induced by Gy, regulating transduction of Wnt/β-catenin signaling. In addition, PER3 contributed to the sensitivity of drug-resistant GC cells to cisplatin.

Conclusion: Period circadian regulator 3 enhances the sensitivity of GC to radiation through Wnt/β-catenin signaling pathway, providing a potential therapeutic strategy for the management of GC.

Keywords: Gastric cancer, Radiation sensitivity, Radiotherapy, PER3, Wnt, β-catenin